Sauer B
National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Curr Opin Biotechnol. 1994 Oct;5(5):521-7. doi: 10.1016/0958-1669(94)90068-x.
Site-specific DNA recombination systems have considerable utility in manipulating DNA and can facilitate many cloning and gene transfer techniques. The ability of a number of recombinases to catalyze efficient DNA recombination in higher eukaryotes has important and exciting consequences for precise chromosome and transgene engineering. Exploitation of these recombinases will facilitate the generation of transgenic animal disease models and help elucidate the function of developmental genes.
位点特异性DNA重组系统在DNA操作方面具有相当大的用途，并且可以促进许多克隆和基因转移技术。多种重组酶在高等真核生物中催化高效DNA重组的能力，对于精确的染色体和转基因工程具有重要且令人兴奋的意义。利用这些重组酶将有助于生成转基因动物疾病模型，并有助于阐明发育基因的功能。
