Ohshiro T, Yamamoto M, Izumi Y, Bui B T, Florentin D, Marquet A
Department of Biotechnology, Faculty of Engineering, Tottori University, Japan.
Biosci Biotechnol Biochem. 1994 Sep;58(9):1738-41. doi: 10.1271/bbb.58.1738.
The activity of biotin synthase, responsible for biotin synthesis from dethiobiotin, was demonstrated in a completely defined reaction mixture with cell-free extracts of a Bacillus sphaericus bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine was active, while L-methionine and L-cysteine had no significant effect. Protein concentrations higher than 15 mg/ml in the reaction mixture were needed to detect biotin synthase activity. When dialyzed cell-free extracts were used for the reaction, NADH, NADPH, or FAD among the well-known cofactors tested enhanced the activity, and Fe2+, Mn2+, and Ca2+ among the metal ions tested also had some effects.
