从班克松发育中的木质部中纯化一种酸性针叶树苷水解β-葡萄糖苷酶

Purification of an acidic coniferin-hydrolysing beta-glucosidase from developing xylem of Pinus banksiana.

作者信息

Leinhos V, Udagama-Randeniya P V, Savidge R A

机构信息

FSU Jena, Institut für Ernährung und Umwelt, Germany.

出版信息

Phytochemistry. 1994 Sep;37(2):311-5. doi: 10.1016/0031-9422(94)85053-4.

DOI:10.1016/0031-9422(94)85053-4

PMID:7765617

Abstract

Two cytosolic, acidic (pI 3.8) glycoproteins (M(r) 110,000 and 90,000) from lignifying xylem of Pinus banksiana were electrophoretically isolated and confirmed by combined gas chromatography-mass spectrometry to be capable of hydrolysing E-coniferin to trans-coniferyl alcohol. These isoenzymes yielded identical N-terminal sequences having negligible homology with documented glucosidases, suggesting that they constitute a novel form of beta-glucosidase.

摘要

从班克松木质化木质部中通过电泳分离出两种胞质酸性糖蛋白（分子量分别为110,000和90,000，等电点为3.8），经气相色谱-质谱联用分析证实它们能够将E-松柏苷水解为反式松柏醇。这些同工酶产生相同的N端序列，与已报道的糖苷酶的同源性可忽略不计，表明它们构成了一种新型的β-葡萄糖苷酶。