Saito K
Radioisotope Research Centre, Kyoto University, Japan.
Phytochemistry. 1994 Nov;37(4):1017-22. doi: 10.1016/s0031-9422(00)89520-5.
Transfer of 3H from D-gluconic acid, specifically labelled with 3H at C-2 or C-3 and 14C at C-1, C-2, or C-3, 4, to L(+)-tartaric acid was examined in leaves and berries of Vitis labrusca cv Delaware and in leaves of Parthenocissus quinquefolia. 3H located at C-3 of D-gluconic acid was highly conserved in this transfer, yielding a 3H/14C ratio between 3.3 and 14 in the light and between 11 and 22 in the dark. These experiments strongly suggest that a portion of the 3H present in L(+)-tartaric acid may have been transferred from D-gluconic acid to L(+)-tartaric acid, possibly via NADP[3H] through a redox process involving reduction of L-xylo-2-hexulosonate (2-keto-L-idonate). Both [3H]-tartaric acid and [14C]tartaric acid synthesized in grape leaves from D-[3-3H, 2-14C]gluconic acid, or [3-3H, 3,4-14C]gluconic acid were characterized as L(+)-chiral form exclusively, the naturally occurring from of tartaric acid.
在美洲葡萄品种特拉华的叶片和浆果以及五叶地锦的叶片中，研究了将D-葡萄糖酸（在C-2或C-3位置用³H特异性标记，在C-1、C-2或C-3、4位置用¹⁴C标记）中的³H转移至L(+)-酒石酸的情况。D-葡萄糖酸C-3位置上的³H在这种转移过程中高度保守，在光照下³H/¹⁴C比值在3.3至14之间，在黑暗中在11至22之间。这些实验有力地表明，L(+)-酒石酸中存在的一部分³H可能是从D-葡萄糖酸转移至L(+)-酒石酸的，可能是通过NADP[³H]，经由涉及L-木糖-2-己酮糖酸（2-酮-L-艾杜糖酸）还原的氧化还原过程。在葡萄叶片中由D-[3-³H, 2-¹⁴C]葡萄糖酸或[3-³H, 3,4-¹⁴C]葡萄糖酸合成的[³H]-酒石酸和[¹⁴C]酒石酸均被明确鉴定为仅为L(+)-手性形式，即酒石酸的天然存在形式。
