Errea M I, Matulewicz M C
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Phytochemistry. 1994 Nov;37(4):1075-8. doi: 10.1016/s0031-9422(00)89531-x.
Cold water extraction of tetrasporic Pterocladia capillacea led to the isolation of a product which was characterized and further treated with Cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration and six fractions were separated. The two major products obtained, one soluble in Cetrimide solution, the other soluble in 0.5 M sodium chloride, were subjected to structural studies, carried out by methylation analysis and 13C NMR spectroscopy. The results obtained indicated for the former a backbone mainly constituted by alternating 3-linked beta-D-galactose units and 4-linked 3,6-anhydro-alpha-L-galactose residues, but with other structural features probably responsible for its non-gelling properties. The acidic fraction is a complex polysaccharide sulphated mainly on C-6 of the beta-D-galactose units and on C-3 of the alpha-L-galactose residues.
对四孢子鸡毛菜进行冷水提取，得到一种产物，对其进行表征后，再用西曲溴铵处理。将络合后的物质在氯化钠浓度不断增加的溶液中进行分级溶解，分离出六个级分。对得到的两种主要产物，一种可溶于西曲溴铵溶液，另一种可溶于0.5 M氯化钠溶液，进行了结构研究，采用甲基化分析和13C核磁共振光谱法。所得结果表明，前者的主链主要由交替的3-连接的β-D-半乳糖单元和4-连接的3,6-脱水-α-L-半乳糖残基组成，但还有其他结构特征可能导致其不具有凝胶特性。酸性级分是一种复合多糖，主要在β-D-半乳糖单元的C-6位和α-L-半乳糖残基的C-3位硫酸化。
