Olasupo N A, Schillinger U, Franz C M, Holzapfel W H
Department of Botany and Microbiology, Lagos State University, Nigeria.
Lett Appl Microbiol. 1994 Dec;19(6):438-41. doi: 10.1111/j.1472-765x.1994.tb00976.x.
An Enterococcus faecium strain from Nigerian fermented skimmed cow milk ('wara') produced bacteriocin inhibitory towards Lactobacillus, Enterococcus and Listeria strains. The bacteriocin (designated enterocin 01) was inactivated by proteases, heat-stable at 100 degrees c and active at pH 2.0-6.0. The Ent. faecium isolate harboured plasmids of ca 36.3 and 23.1 kb. Curing experiments with ethidium bromide resulted in a bacteriocin-negative mutant which had not lost immunity to the bacteriocin. Slight differences in plasmid profiles between wild-type and mutant indicated a possible plasmid-coded bacteriocin production.
从尼日利亚发酵脱脂牛奶（“wara”）中分离出的一株粪肠球菌产生了对乳酸杆菌、肠球菌和李斯特菌具有抑制作用的细菌素。该细菌素（命名为肠球菌素01）可被蛋白酶灭活，在100℃下热稳定，在pH 2.0 - 6.0时具有活性。该粪肠球菌分离株携带约36.3 kb和23.1 kb的质粒。用溴化乙锭进行的消除实验产生了一个细菌素阴性突变体，该突变体并未丧失对细菌素的免疫性。野生型和突变体之间质粒图谱的细微差异表明细菌素的产生可能由质粒编码。
