Peeters A J, Proveniers M, van Hoek A, Schreuder M, Gerards W, Barendse G W, Wullems G J
Department of Experimental Botany, University of Nijmegen, The Netherlands.
Planta. 1994;195(2):271-81. doi: 10.1007/BF00199687.
The development of vegetative and generative buds on thin-layer explants of tobacco (Nicotiana tabacum L. cv. Samsun) has been studied at the level of translatable mRNA to detect changes in the mRNA population during bud initiation and differentiation, and several quantitative differences were found. By differential screening of a cDNA library obtained from flower-bud-regenerating explants we have isolated a group of six cDNA clones representing genes that are preferentially expressed during in-vitro flower bud formation. Nucleotide sequence analysis of one of these cDNAs, pAP8, showed that the most likely open reading frame has some typical characteristics of, and homology with, extensin-like genes. Northern blot analysis and in-situ hybridization suggest a specific role for these extensin-like genes in flower bud initiation on tobacco pedicel explants.
为了检测芽起始和分化过程中mRNA群体的变化，我们在可翻译的mRNA水平上研究了烟草（Nicotiana tabacum L. cv. Samsun）薄层外植体上营养芽和生殖芽的发育，并发现了一些数量上的差异。通过对从花芽再生外植体获得的cDNA文库进行差异筛选，我们分离出一组六个cDNA克隆，它们代表了在体外花芽形成过程中优先表达的基因。对其中一个cDNA pAP8进行核苷酸序列分析表明，最可能的开放阅读框具有一些类伸展蛋白基因的典型特征并与之具有同源性。Northern印迹分析和原位杂交表明，这些类伸展蛋白基因在烟草花梗外植体的花芽起始中具有特定作用。
