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Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus brevis.

作者信息

Louw M E, Reid S J, James D M, Watson T G

机构信息

Biotechnology Programme, Division of Food Science and Technology, CSIR, Pretoria, Republic of South Africa.

出版信息

Appl Microbiol Biotechnol. 1994 Oct;42(1):78-84. doi: 10.1007/BF00170228.

DOI:10.1007/BF00170228
PMID:7765823
Abstract

The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec- strain as well as in a B. subtilis sacU(HY) strain.

摘要

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Cloning and characterization of Bacillus subtilis iep, which has positive and negative effects on production of extracellular proteases.枯草芽孢杆菌iep的克隆与特性分析，该基因对胞外蛋白酶的产生具有正负两方面影响。
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