Kolibachuk D, Baumann P
Microbiology Section, University of California, Davis 95616-8665.
Curr Microbiol. 1995 Mar;30(3):133-6. doi: 10.1007/BF00296197.
Buchnera aphidicola is an endosymbiont of the aphid Schizaphis graminum. A 3.9-kb B. aphidicola DNA fragment was sequenced and found to contain two open reading frames (ORFs). The deduced amino acid sequence of one of the ORFs had an 85% identity to Escherichia coli glyceraldehyde-3-phosphate dehydrogenase (Gap). Both of these proteins have a higher similarity to eukaryotic than to prokaryotic Gaps. The second ORF could not be readily identified. The sequence of the putative product indicated that it was a member of the family of ATP-binding, membrane-associated proteins. The highest amino acid identity (36%) was with E. coli FtsE, a protein involved in cell division.
蚜虫内共生菌是麦二叉蚜的一种内共生体。对一段3.9千碱基的蚜虫内共生菌DNA片段进行了测序，发现其包含两个开放阅读框（ORF）。其中一个ORF推导的氨基酸序列与大肠杆菌甘油醛-3-磷酸脱氢酶（Gap）有85%的同一性。这两种蛋白质与真核生物的Gap的相似性高于与原核生物Gap的相似性。第二个ORF不容易被识别。推测产物的序列表明它是ATP结合、膜相关蛋白家族的一员。最高的氨基酸同一性（36%）是与大肠杆菌FtsE，一种参与细胞分裂的蛋白质。
