沙丁鱼降钙素的氨基酸序列及其在大鼠体内的降钙活性。

Amino acid sequence of sardine calcitonin and its hypocalcemic activity in rats.

作者信息

Suzuki N, Nosé Y, Kasé Y, Sasayama Y, Takei Y, Nagasawa H, Watanabe T X, Nakajima K, Sakakibara S

机构信息

Department of Biology, Faculty of Science, Toyama University, Japan.

出版信息

Zoolog Sci. 1994 Oct;11(5):713-6.

PMID:7765857

Abstract

A novel calcitonin (CT) was isolated from the spotlined sardine, Sardinops melanostictus. The primary structure of sardine CT was determined as follows: H-Cys-Ser-Asn-Leu-Ser-Thr- Cys-Ala-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Ser-Tyr-Pro-Arg- Thr- Asn-Val-Gly-Ala-Gly-Thr-Pro-NH2. This amino acid sequence was different from that of salmon CT in 4 amino acid residues at positions 8th, 21th, 27th and 29th. As judged by the international method of CT bioassay, hypocalcemic activity of sardine CT was calculated as 4156 IU/mg. When compared for durability of CTs, it was found that sardine CT was significantly more potent than that of salmon CT. This is the first report of CT from a marine species of teleost.

摘要

从斑头沙丁鱼（Sardinops melanostictus）中分离出一种新型降钙素（CT）。沙丁鱼CT的一级结构确定如下：H-半胱氨酸-丝氨酸-天冬酰胺-亮氨酸-丝氨酸-苏氨酸-半胱氨酸-丙氨酸-亮氨酸-甘氨酸-赖氨酸-亮氨酸-丝氨酸-谷氨酰胺-谷氨酸-亮氨酸-组氨酸-赖氨酸-亮氨酸-谷氨酰胺-丝氨酸-酪氨酸-脯氨酸-精氨酸-苏氨酸-天冬酰胺-缬氨酸-甘氨酸-丙氨酸-甘氨酸-苏氨酸-脯氨酸-NH2。该氨基酸序列在第8、21、27和29位的4个氨基酸残基上与鲑鱼CT不同。根据国际CT生物测定法判断，沙丁鱼CT的降钙活性计算为4156 IU/mg。在比较CT的耐久性时，发现沙丁鱼CT比鲑鱼CT的效力明显更强。这是关于硬骨鱼海洋物种CT的首次报道。