Lu P, Marquardt R R, Kierek-Jaszczuk D
Department of Animal Science, University of Manitoba, Winnipeg, Canada.
Lett Appl Microbiol. 1995 Jan;20(1):41-5. doi: 10.1111/j.1472-765x.1995.tb00403.x.
Aspergillus ochraceus Wilhelm is a producer of highly toxic mycotoxin, ochratoxin A, and is known to contribute to the damage of grain on storage. Rabbit antibodies were produced against the exoantigens of A. ochraceus, and ELISA and immunoblotting procedures were used to characterize their reactivity towards fungal exoantigens of four Aspergillus, seven Penicillium, three Fusarium, one Mucor and one Alternaria species. The antibodies were specific for A. ochraceus and essentially cross-reacted neither with other fungi nor with the water-soluble grain components. These antibodies, therefore, have the potential of being utilized for the discriminative monitoring of A. ochraceus in foodstuffs and feeds.
棕曲霉Wilhelm是一种剧毒霉菌毒素赭曲霉毒素A的产生菌，已知会对储存的谷物造成损害。制备了针对棕曲霉外抗原的兔抗体，并采用酶联免疫吸附测定（ELISA）和免疫印迹法来表征其对四种曲霉、七种青霉、三种镰刀菌、一种毛霉和一种链格孢菌的真菌外抗原的反应性。这些抗体对棕曲霉具有特异性，基本上既不与其他真菌发生交叉反应，也不与水溶性谷物成分发生交叉反应。因此，这些抗体有潜力用于鉴别监测食品和饲料中的棕曲霉。
