Branquinha M H, Meirelles M N, Lopes A, Moreira C, Vermelho A B
Departamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Brasil.
Curr Microbiol. 1995 Feb;30(2):77-82. doi: 10.1007/BF00294186.
Glycoconjugates from five trypanosomatid genera--Crithidia, Herpetomonas, Endotrypanum, Leishmania, and Trypanosoma--were extracted with Triton X-114 and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by periodic acid-Schiff staining. Most of the glycoconjugates were detected in the hydrophobic phase, indicating the presence of anchored glycoconjugates. All the trypanosomatids expressed a glycoconjugate with a low molecular weight (below 20 kDa) in this phase. In each species, however, a characteristic and specific pattern of glycoconjugates was also observed in both phases. In the hydrophobic phase: 14-29 kDa glycoconjugates in C. guilhermei; 24-70 kDa in C. fasciculata, C. luciliae, E. schaudinni, and T. cruzi Y and G strains; 45-66 kDa in C. oncopelti and H. samuelpessoai; above 36 kDa in T. dionisii; 20-24 kDa, 36-45 kDa, and 70 kDa in L. tarentolae and T. mega. In the hydrophilic phase, typical glycoproteins were observed in some trypanosomatids: 60 kDa in T. mega and T. cruzi Y strain; 70 kDa in H. samuelpessoai; 66 kDa in C. oncopelti; 20-70 kDa in C. luciliae. These findings suggest that Triton X-114-extracted glycoconjugates could be useful markers for trypanosomatid taxonomy.
从五个锥虫属——克氏锥虫属、动基体虫属、内锥虫属、利什曼原虫属和锥虫属——中提取糖缀合物，用Triton X-114进行萃取，并通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳，随后进行过碘酸-希夫染色分析。大多数糖缀合物在疏水相中被检测到，表明存在锚定糖缀合物。在该相中，所有锥虫均表达一种低分子量（低于20 kDa）的糖缀合物。然而，在每个物种中，在两个相中也观察到了糖缀合物的特征性和特异性模式。在疏水相中：圭氏克氏锥虫中有14 - 29 kDa的糖缀合物；fasiculata克氏锥虫、luciliae克氏锥虫、schaudinni内锥虫以及克氏锥虫Y和G菌株中有24 - 70 kDa的糖缀合物；oncopelti克氏锥虫和samuelpessoai动基体虫中有45 - 66 kDa的糖缀合物；dionisii锥虫中有高于36 kDa的糖缀合物；tarentolae利什曼原虫和巨型锥虫中有20 - 24 kDa、36 - 45 kDa和70 kDa的糖缀合物。在亲水相中，在一些锥虫中观察到了典型的糖蛋白：巨型锥虫和克氏锥虫Y菌株中有60 kDa的糖蛋白；samuelpessoai动基体虫中有70 kDa的糖蛋白；oncopelti克氏锥虫中有66 kDa的糖蛋白；luciliae克氏锥虫中有20 - 70 kDa的糖蛋白。这些发现表明，Triton X-114提取的糖缀合物可能是锥虫分类学的有用标记。
