采用夹心酶联免疫吸附测定法检测大豆制品中主要大豆过敏原Gly m Bd 30K的含量。

Measurement of Gly m Bd 30K, a major soybean allergen, in soybean products by a sandwich enzyme-linked immunosorbent assay.

作者信息

Tsuji H, Okada N, Yamanishi R, Bando N, Kimoto M, Ogawa T

机构信息

Department of Nutrition, School of Medicine, University of Tokushima, Japan.

出版信息

Biosci Biotechnol Biochem. 1995 Jan;59(1):150-1. doi: 10.1271/bbb.59.150.

DOI:10.1271/bbb.59.150

PMID:7765969

Abstract

By a sandwich enzyme-linked immunosorbent assay, a soybean major allergen, Gly m Bd 30K, in soybean products was measured. The allergen occurred at high concentrations in soy milk, tofu, kori-dofu, and yuba, but its content in kinako was small. No allergen was found in fermented foods such as miso, shoyu, and natto. The allergen was clearly shown to occur in meat balls, beef croquettes, and fried chicken that contained soybean protein isolate.

摘要

通过夹心酶联免疫吸附测定法，对豆制品中的大豆主要过敏原Gly m Bd 30K进行了检测。该过敏原在豆浆、豆腐、冻豆腐和腐竹中含量很高，但在黄豆粉中的含量较少。在味噌、酱油和纳豆等发酵食品中未发现该过敏原。在含有大豆分离蛋白的肉丸、牛肉饼和炸鸡中，该过敏原也有明显检出。