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Isolation, sequence and expression in Escherichia coli of the nitrite reductase gene from the filamentous, thermophilic cyanobacterium Phormidium laminosum.

作者信息

Merchán F, Prieto R, Kindle K L, Llama M J, Serra J L, Fernández E

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Spain.

出版信息

Plant Mol Biol. 1995 Mar;27(5):1037-42. doi: 10.1007/BF00037030.

DOI:10.1007/BF00037030
PMID:7766873
Abstract

The nitrite reductase (NiR) gene (nirA) has been isolated and sequenced from the filamentous, thermophilic non-N2-fixing cyanobacterium Phormidium laminosum. Putative promoter-like and Shine-Dalgarno sequences appear at the 5' end of the 1533 bp long nir-coding region. The deduced amino acid sequence of NiR from P. laminosum corresponds to a 56 kDa polypeptide, a size identical to the molecular mass previously determined for the pure enzyme, and shows a high identity with amino acid sequences from ferredoxin-dependent NiR. This cyanobacterial NiR gene has been efficiently expressed in Escherichia coli DH5 alpha from the E. coli lac promoter and probably from the P. laminosum NiR promoter.

摘要

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本文引用的文献

1
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Plant Physiol. 1988 Nov;88(3):741-6. doi: 10.1104/pp.88.3.741.
2
Nitrite reductase gene from Synechococcus sp. PCC 7942: homology between cyanobacterial and higher-plant nitrite reductases.来自聚球藻属PCC 7942的亚硝酸还原酶基因：蓝藻和高等植物亚硝酸还原酶之间的同源性
Plant Mol Biol. 1993 Mar;21(6):1201-5. doi: 10.1007/BF00023618.
3
Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942.
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聚球藻属蓝细菌Synechococcus sp. PCC7942中参与硝酸盐转运的基因簇的鉴定与表征。
Mol Gen Genet. 1993 Jan;236(2-3):193-202. doi: 10.1007/BF00277112.
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A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
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