Dette C, Wätzig H
Institute of Pharmacy and Food Chemistry, University of Würzburg, Germany.
J Chromatogr A. 1995 May 12;700(1-2):89-94. doi: 10.1016/0021-9673(94)01261-c.
The thrombin inhibitor r-hirudin is a peptide of 65 amino acids and with a pI of 4.4. Capillary electrophoresis (CE) was used to separate r-hirudin from seven closely related substances which may be found as by-products or from degradation. Possibly two of these substances differ only in an isoaspartyl instead of an aspartyl binding. A baseline separation was possible with an acetate buffer (pH 4.4, 60 mM) containing 0.3% (m/m) PEG 20,000 and 0.1 mM Zn2+. The possibilities to prevent wall adsorption are discussed.
凝血酶抑制剂重组水蛭素是一种由65个氨基酸组成的肽，等电点为4.4。采用毛细管电泳（CE）从七种可能作为副产物或降解产物存在的密切相关物质中分离重组水蛭素。其中可能有两种物质仅在异天冬氨酰而非天冬氨酰结合上存在差异。使用含有0.3%（m/m）聚乙二醇20000和0.1 mM锌离子的醋酸盐缓冲液（pH 4.4，60 mM）可以实现基线分离。文中还讨论了防止壁吸附的可能性。
