大鼠嗜铬细胞瘤细胞系PC12中血管活性肠肽基因的表达

Vasoactive intestinal peptide gene expression in the rat pheochromocytoma cell line PC12.

作者信息

Tsukada T, Fukushima M, Takebe H, Nakai Y

机构信息

Department of Radiation Genetics, Kyoto University Faculty of Medicine, Japan.

出版信息

Mol Cell Endocrinol. 1995 Feb;107(2):231-9. doi: 10.1016/0303-7207(94)03448-3.

DOI:10.1016/0303-7207(94)03448-3

PMID:7768335

Abstract

Vasoactive intestinal peptide (VIP) gene expression was analyzed in PC12 cells. VIP mRNA was detected in PC12 cells treated with VIP or forskolin whereas no VIP mRNA was detected in the untreated cells. The induction of the VIP mRNA was enhanced by the simultaneous treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). PC12 cells stimulated with forskolin plus TPA released immunoreactive VIP. Sephadex G-50 column chromatography revealed that the immunoreactive VIP secreted from PC12 cells is comprised of multiple forms, one of which was indistinguishable from the authentic VIP. PC12 cells supported an efficient transcription from the human VIP gene promoter in a cell-specific as well as cAMP-dependent manner. These results definitely demonstrated the expression of the VIP gene in PC12 cells. VIP biosynthesis may be positively regulated by VIP in an autocrine fashion in PC12 cells.

摘要

在PC12细胞中分析了血管活性肠肽（VIP）基因的表达。在用VIP或福斯高林处理的PC12细胞中检测到VIP mRNA，而在未处理的细胞中未检测到VIP mRNA。同时用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）处理可增强VIP mRNA的诱导。用福斯高林加TPA刺激的PC12细胞释放免疫反应性VIP。葡聚糖G - 50柱色谱显示，PC12细胞分泌的免疫反应性VIP由多种形式组成，其中一种与天然VIP无法区分。PC12细胞以细胞特异性以及cAMP依赖性方式支持人VIP基因启动子的有效转录。这些结果明确证明了VIP基因在PC12细胞中的表达。在PC12细胞中，VIP生物合成可能以自分泌方式受到VIP的正向调节。