Lubeck M D, Johnson F B
Infect Immun. 1976 Apr;13(4):1289-92. doi: 10.1128/iai.13.4.1289-1292.1976.
Bovine parvovirus has been found to infect two cell strains: buffalo lung fibroblasts and embryonic bovine tracheal cells. Infectivity titers were best determined by plaque assays. Immunofluorescence studies revealed intranuclear staining, whereas complement fixation tests confirmed bovine parvovirus antigen production. These cell strains represent economical and convenient cell types which can be used in studies on bovine parvovirus.
水牛肺成纤维细胞和牛胚胎气管细胞。感染性滴度最好通过空斑试验来测定。免疫荧光研究显示细胞核内染色,而补体结合试验证实了牛细小病毒抗原的产生。这些细胞株是经济且方便的细胞类型,可用于牛细小病毒的研究。