一种快速的神经氨酸酶检测方法。检测与病毒转化相关的两种活性差异。

A rapid assay for neuraminidase. The detection of two differences in activity associated with virus transformation.

作者信息

Santer U V, Yee-Foon J, Glick M C

出版信息

Biochim Biophys Acta. 1978 Apr 12;523(2):435-42. doi: 10.1016/0005-2744(78)90046-3.

Abstract

Neuraminidase (acylneuraminyl hydrolase, EC 3.2.1.18) activity in fibroblast homogenates was measured by a rapid and simple assay with a synthetic substrate. The activity of neuraminidase in virus transformed hamster fibroblasts was increased over the normal counterpart. In addition, the differential activity seen using the synthetic substrate and fetuin made it possible to detect an enzyme activity hitherto not described. The advantages of this assay for metabolic screening are discussed.

摘要

通过使用合成底物的快速简便测定法，测定成纤维细胞匀浆中的神经氨酸酶（酰基神经氨酸水解酶，EC 3.2.1.18）活性。病毒转化的仓鼠成纤维细胞中神经氨酸酶的活性高于正常细胞。此外，使用合成底物和胎球蛋白观察到的差异活性使得检测一种迄今未描述的酶活性成为可能。本文讨论了该测定法在代谢筛查中的优势。