Hashimoto N, Watanabe T, Shiratori Y, Ikeda Y, Kato H, Han K, Yamada H, Toda G, Kurokawa K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Hepatology. 1995 Jun;21(6):1713-8.
We investigated the secretory profiles of prostanoids in two types of nonparenchymal cell from the rat liver, sinusoidal endothelial cells and Kupffer cells, in primary culture both under basal conditions and after stimulation with adenine nucleotides. Prostaglandin (PG) E2 was the main prostanoid secreted by both types of hepatic nonparenchymal cell in the basal and adenosine triphosphate (ATP)-stimulated states. Time- and concentration-dependent effects of ATP-mediated PGE2 secretion were noted in sinusoidal endothelial cells, whereas the profile of the relative potencies of individual nucleotides was consistent with the presence of P2y and P1 purinergic receptors. In Kupffer cells, the regulation of prostanoid secretion by adenine nucleotides was essentially the same as that in sinusoidal endothelial cells except that adenosine did not stimulate prostanoid secretion and that prostanoid secretion differed somewhat; Kupffer cells secreted relatively more PGF2 alpha and less 6-keto-PGF1 alpha than sinusoidal endothelial cells in the presence of ATP, suggesting the presence of only P2y receptors. In summary, PGE2 is the main prostanoid secreted by hepatic nonparenchymal cells and its secretion may be stimulated by adenine nucleotides and adenosine.
我们研究了原代培养的大鼠肝脏两种非实质细胞，即窦状内皮细胞和库普弗细胞，在基础条件下以及用腺嘌呤核苷酸刺激后前列腺素类物质的分泌情况。在基础状态和三磷酸腺苷（ATP）刺激状态下，前列腺素（PG）E2都是这两种肝脏非实质细胞分泌的主要前列腺素类物质。在窦状内皮细胞中观察到了ATP介导的PGE2分泌的时间和浓度依赖性效应，而单个核苷酸的相对效力情况与P2y和P1嘌呤能受体的存在一致。在库普弗细胞中，腺嘌呤核苷酸对前列腺素类物质分泌的调节与窦状内皮细胞基本相同，只是腺苷不刺激前列腺素类物质分泌，且前列腺素类物质分泌有所不同；在存在ATP的情况下，库普弗细胞分泌的PGF2α相对较多，6-酮-PGF1α相对较少，这表明只存在P2y受体。总之，PGE2是肝脏非实质细胞分泌的主要前列腺素类物质，其分泌可能受到腺嘌呤核苷酸和腺苷的刺激。
