单细胞蓝藻聚球藻属6803菌株染色体的物理图谱和遗传图谱。
Physical and genetic map of the chromosome of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803.
作者信息
Churin Y N, Shalak I N, Börner T, Shestakov S V
机构信息
Department of Genetics, Humboldt University Berlin, Germany.
出版信息
J Bacteriol. 1995 Jun;177(11):3337-43. doi: 10.1128/jb.177.11.3337-3343.1995.
Abstract
A combined physical and genetic map of the cyanobacterium Synechocystis sp. strain PCC 6803 chromosome was constructed. An estimated genome size of 3.82 Mb was obtained by summing the sizes of 25 MluI or 40 NotI fragments seen by pulsed-field electrophoresis. The order of the restriction fragments was determined by using two independent experimental approaches: pulsed-field fragment hybridization and linking clone analysis. The relative positions of 30 known genes or gene clusters were localized.
摘要
构建了集胞藻6803菌株染色体的物理图谱与遗传图谱。通过脉冲场电泳观察到的25个MluI片段或40个NotI片段大小相加，得出估计的基因组大小为3.82 Mb。利用两种独立的实验方法确定了限制性片段的顺序：脉冲场片段杂交和连接克隆分析。定位了30个已知基因或基因簇的相对位置。
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