Lim I K, Lee M S, Lee S H, Kim N K, Jou I, Seo J S, Park S C
Department of Biochemistry, School of Medicine, Ajou University, Suwon, Korea.
J Cancer Res Clin Oncol. 1995;121(5):279-84. doi: 10.1007/BF01209594.
As a part of a series of investigations on the functions of TIS21 and TIS1 genes, we measured in vivo 12-O-tetradecanoylphorbol-13-acetate (TPA) inducibility of primary response genes (TIS21, TIS8 and TIS1) in the Balb/c mice and the changes of TIS gene expression in thymic carcinoma tissues and A549 and NCIH69 human lung cancer cell lines. In vivo induction of the TIS genes (TIS21, -8 and -1) by intraperitoneal injection of TPA was dramatic only at the needle contact site, i.e. in the abdominal muscle, not in the thigh muscle. Expression of TIS21 and TIS1 in the Balb/c mice thymus, lung, stomach and spleen was very strong (Lim IK et al. 1994a), regardless of TPA injection. Thymic carcinoma tissues developed in SV40-T-antigen-containing transgenic mice did not express TIS21 and TIS1, and expressed TIS8 weakly. Interestingly, induction of TIS21 expression was obliterated in the human lung cancer cells; A549 cells completely lost the ability to express TIS21 after a combined treatment of TPA and cycloheximide. We also measured the induction of TIS genes by TPA and/or cycloheximide in Raw264.7 mouse macrophage cells and U937 human histiocytic lymphoma cells. However, the induction profile was quite different; repressed and deregulated expression in the U937 cells as compared to rapid and transient induction of TIS genes in the Raw264.7 cells. These data may suggest a repressed expression of TIS21 and TIS1 in the cancer tissues and cells derived from the organs that constitutively express TIS21 in mice and in human cancer cells.
作为对TIS21和TIS1基因功能进行的一系列研究的一部分，我们在Balb/c小鼠体内测量了初级反应基因（TIS21、TIS8和TIS1）对12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）的诱导性，以及胸腺癌组织、A549和NCIH69人肺癌细胞系中TIS基因表达的变化。通过腹腔注射TPA在体内诱导TIS基因（TIS21、-8和-1），仅在注射针接触部位即腹肌中显著，而在大腿肌肉中不显著。无论是否注射TPA，TIS21和TIS1在Balb/c小鼠的胸腺、肺、胃和脾脏中的表达都很强（Lim IK等人，1994a）。在含SV40 - T - 抗原的转基因小鼠中发生的胸腺癌组织不表达TIS21和TIS1，而TIS8表达较弱。有趣的是，人肺癌细胞中TIS21表达的诱导被消除；在TPA和环己酰亚胺联合处理后，A549细胞完全丧失了表达TIS21的能力。我们还测量了TPA和/或环己酰亚胺在Raw264.7小鼠巨噬细胞和U937人组织细胞淋巴瘤细胞中对TIS基因的诱导。然而，诱导模式有很大不同；与Raw264.7细胞中TIS基因的快速和短暂诱导相比，U937细胞中的表达受到抑制且失调。这些数据可能表明在源自小鼠中组成性表达TIS21的器官的癌组织和细胞以及人癌细胞中，TIS21和TIS1表达受到抑制。
