Even C, Plagemann P G
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455, USA.
J Virol. 1995 Jul;69(7):4237-44. doi: 10.1128/JVI.69.7.4237-4244.1995.
Infection of cultures of peritoneal macrophages with both lactate dehydrogenase-elevating virus (LDV) and mouse hepatitis virus (MHV) resulted in the formation of pseudotype virions containing LDV RNA which productively infected cells that are resistant to infection by intact LDV virions but not to infection by MHV. These cells were mouse L-2 and 3T3-17Cl-1 cells as well as residual peritoneal macrophages from persistently LDV-infected mice. Productive LDV infection of these cells via pseudotype virions was inhibited by antibodies to the MHV spike protein or to the MHV receptor, indicating that LDV RNA entered the cells via particles containing the MHV envelope. Simultaneous exposure of L-2 cells to both LDV and MHV resulted in infection by MHV but not by LDV. The results indicate that an internal block to LDV replication is not the cause of the LDV nonpermissiveness of many cell types, including the majority of the macrophages in an adult mouse. Instead, LDV permissiveness is restricted to a subpopulation of mouse macrophages because only these cells possess a surface component that acts as an LDV receptor.
用乳酸脱氢酶升高病毒（LDV）和小鼠肝炎病毒（MHV）感染腹膜巨噬细胞培养物，导致形成含有LDV RNA的假型病毒粒子，这些假型病毒粒子能有效感染对完整LDV病毒粒子感染具有抗性但对MHV感染不具有抗性的细胞。这些细胞包括小鼠L-2和3T3-17Cl-1细胞以及来自持续感染LDV的小鼠的残余腹膜巨噬细胞。通过假型病毒粒子对这些细胞进行有效的LDV感染受到针对MHV刺突蛋白或MHV受体的抗体的抑制，这表明LDV RNA通过含有MHV包膜的颗粒进入细胞。同时将L-2细胞暴露于LDV和MHV会导致MHV感染而非LDV感染。结果表明，对LDV复制的内部阻断不是许多细胞类型（包括成年小鼠中的大多数巨噬细胞）对LDV不敏感的原因。相反，LDV的易感性仅限于小鼠巨噬细胞的一个亚群，因为只有这些细胞具有作为LDV受体的表面成分。
