Lund T, Medveczky M M, Geck P, Medveczky P G
Department of Microbiology and Immunology, University of South Florida, Tampa 33612-4799, USA.
J Virol. 1995 Jul;69(7):4495-9. doi: 10.1128/JVI.69.7.4495-4499.1995.
A region of the herpesvirus saimiri genome encoding an mRNA with two open reading frames (ORFs) has been identified to be essential for transformation of T cells. Deletion of either ORF resulted in the loss of transforming ability. ORF-1 has been shown to code for a collagen-like oncoprotein. This study shows for the first time that the bicistronic mRNA can translate a 32-kDa protein from ORF-2. Polyclonal serum to ORF-2 was generated by using a glutathione fusion protein. Using this antiserum, ORF-2 was localized in cell membranes and is expressed on the outer cell membrane. The half-life of this membrane protein was found to be about 5.5 h. Limited sequence similarity was found between ORF-2 and interleukin-11; however, no secretion of ORF-2 protein was detected in supernatants from transformed cells. Further studies are required to investigate the potential interaction with the interleukin-11 receptor.
已确定猴疱疹病毒基因组中编码一种带有两个开放阅读框（ORF）的mRNA的区域对T细胞转化至关重要。删除任何一个ORF都会导致转化能力丧失。已证明ORF-1编码一种胶原样癌蛋白。本研究首次表明，双顺反子mRNA可从ORF-2翻译出一种32 kDa的蛋白质。通过使用谷胱甘肽融合蛋白产生了针对ORF-2的多克隆血清。使用这种抗血清，ORF-2定位于细胞膜，并在外细胞膜上表达。发现这种膜蛋白的半衰期约为5.5小时。在ORF-2与白细胞介素-11之间发现了有限的序列相似性；然而，在转化细胞的上清液中未检测到ORF-2蛋白的分泌。需要进一步研究来调查其与白细胞介素-11受体的潜在相互作用。
