Brewster W J, Fernyhough P, Diemel L T, Mohiuddin L, Tomlinson D R
Department of Pharmacology, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, London, UK.
Brain Res Mol Brain Res. 1995 Mar;29(1):131-9. doi: 10.1016/0169-328x(94)00238-a.
This study was designed to explore effects of experimental diabetes mellitus on expression of substance P in the trigeminal ganglion and of nerve growth factor (NGF) in the iris. Rats with streptozotocin-diabetes showed an increase in NGF mRNA in the iris (P < 0.01) which was corrected by insulin treatment. There was also an increase in the levels of mRNA for the substance P precursor, preprotachykinin (PPT), in the trigeminal ganglion of these animals, despite there being no change in GAP-43 mRNA. Since expression of both of these peptides is sensitive to NGF in vitro, we examined the effect of treatment of diabetic rats with NGF at three different doses (0.2, 0.5 and 1.0 mg/kg body weight). There was a dose-dependent increase in both gamma-PPT and GAP-43 mRNA in the trigeminal ganglia of NGF-treated diabetic rats. The findings indicate that increased NGF may be responsible for raised substance P levels in the iris.
本研究旨在探讨实验性糖尿病对三叉神经节中P物质表达及虹膜中神经生长因子（NGF）表达的影响。链脲佐菌素诱导的糖尿病大鼠虹膜中NGF mRNA水平升高（P < 0.01），胰岛素治疗可使其恢复正常。这些动物的三叉神经节中P物质前体、前速激肽原（PPT）的mRNA水平也升高，尽管GAP - 43 mRNA无变化。由于这两种肽在体外的表达均对NGF敏感，我们检测了用三种不同剂量（0.2、0.5和1.0 mg/kg体重）的NGF治疗糖尿病大鼠的效果。NGF治疗的糖尿病大鼠三叉神经节中γ - PPT和GAP - 43 mRNA均呈剂量依赖性增加。研究结果表明，NGF增加可能是虹膜中P物质水平升高的原因。
