Peak J G, Peak M J
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, IL 60439, USA.
Photochem Photobiol. 1995 May;61(5):484-7. doi: 10.1111/j.1751-1097.1995.tb02349.x.
Action spectra (365-520 nm) for the formation of DNA single-strand breaks (SSB) and slowly developing alkali-labile sites (SDALS) in human teratocarcinoma P3 cells in culture were determined. Induction of SDALS results from the absorption of blue- and green-light photons. The spectrum has a broad peak that is maximal between 400 nm to 500 nm and declines sharply above and below these wavelength regions. Negligible yields of SDALS were produced by photons at wavelengths of 365 nm or shorter and at 520 nm or longer, whereas for SSB, the action ioffeases with shorter wavelength throughout the whole spectral range studied. The configuration of the SDALS action spectrum suggests that the primary chromophore, and therefore possibly the photosensitizer, is a mixture of porphyrin and flavin residues.
测定了培养的人畸胎瘤P3细胞中DNA单链断裂（SSB）和缓慢形成的碱不稳定位点（SDALS）形成的作用光谱（365 - 520 nm）。SDALS的诱导是由于蓝光和绿光光子的吸收。该光谱有一个宽峰，在400 nm至500 nm之间最大，在这些波长区域之上和之下急剧下降。在365 nm或更短以及520 nm或更长波长的光子产生的SDALS产量可忽略不计，而对于SSB，在所研究的整个光谱范围内，作用随波长缩短而增加。SDALS作用光谱的形态表明，主要发色团，因此可能的光敏剂，是卟啉和黄素残基的混合物。
