Nagata T
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Cell Mol Biol (Noisy-le-grand). 1995 Feb;41(1):21-38.
For the purpose of analyzing the changes of macromolecular synthesis in aging animals, we have developed methodologies for quantitative light and electron microscopic radioautography. The methodologies were applied to DNA, RNA, protein, glucides and lipids syntheses of digestive organs, especially of the liver and the pancreas of aging strain ddY mice, demonstrating the incorporations of respective precursors, 3H-thymidine, 3H-uridine, 3H-leucine, 3H-glucosamine, 35S-sulfuric acid and 3H-glycerol. Characteristic differences have been observed between the respective macromolecular syntheses in respective aging groups, from prenatal (embryonic day 19) to postnatal newborn period (postnatal day 1), from suckling (postnatal day 3) to weaning (postnatal day 14), juvenile (postnatal 1 month), young adult (postnatal 2 month) and senescent periods (6 and 12 months up to 2 years). These results are extensively analyzed and reviewed.
为了分析衰老动物中大分子合成的变化，我们开发了定量光镜和电镜放射自显影方法。这些方法应用于衰老ddY小鼠消化器官，特别是肝脏和胰腺的DNA、RNA、蛋白质、糖类和脂质合成，证明了各自前体3H-胸腺嘧啶核苷、3H-尿苷、3H-亮氨酸、3H-氨基葡萄糖、35S-硫酸和3H-甘油的掺入。在从产前（胚胎第19天）到产后新生儿期（出生后第1天）、从哺乳期（出生后第3天）到断奶期（出生后第14天）、幼年（出生后1个月）、青年成年期（出生后2个月）和衰老期（6个月、12个月直至2年）的各个衰老组中，观察到了各自大分子合成之间的特征差异。对这些结果进行了广泛的分析和综述。
