通过光镜和电镜放射自显影术研究发育中小鼠卵巢、子宫和输卵管中的核酸合成。
Nucleic acid synthesis in the developing mouse ovary, uterus and oviduct studied by light and electron microscopic radioautography.
作者信息
Li S F, Nagata T
机构信息
Department of Anatomy and Cell Biology, Shinshu University, School of Medicine, Matsumoto, Japan.
出版信息
Cell Mol Biol (Noisy-le-grand). 1995 Feb;41(1):185-95.
The DNA and RNA synthesis in the developing mouse ovary and oviduct were studied by 3H-thymidine and 3H-uridine radioautography. The results showed that both DNA and RNA syntheses were active in all surface epithelial cells, in stromal and follicular cells of the ovaries between postnatal days 1 to 7; then they decreased significantly from day 14 on. However, the DNA synthesis in the epithelial and in the stromal cells of the uteri and oviducts was active at days 1 and 3 and decreased from day 7. The RNA synthesis in the uteri and oviducts was active at postnatal day 1, increased from postnatal day 1 to day 14, decreasing between days 30 to 60. An unparalleled alternation of the DNA and RNA synthesis was shown between the ovary and uterus or oviduct. After postnatal day 3, the RNA synthesis was more important in the uterus and oviduct than in the ovary.
采用³H-胸腺嘧啶核苷和³H-尿苷放射自显影技术，研究了发育中小鼠卵巢和输卵管中的DNA和RNA合成情况。结果显示，在出生后1至7天，卵巢的所有表面上皮细胞、基质细胞和卵泡细胞中的DNA和RNA合成均很活跃；从第14天起，它们显著减少。然而，子宫和输卵管上皮细胞及基质细胞中的DNA合成在第1天和第3天活跃，从第7天起减少。子宫和输卵管中的RNA合成在出生后第1天活跃，从出生后第1天至第14天增加，在第30天至第60天减少。卵巢与子宫或输卵管之间DNA和RNA合成呈现出不同的变化情况。出生后第3天之后，子宫和输卵管中的RNA合成比卵巢中的更为重要。
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