Maurer-Schultze B, Bassukas I D, Hofmockel G
Institute of Medical Radiation and Cell Research, University of Würzburg, Germany.
Cell Mol Biol (Noisy-le-grand). 1995 Feb;41(1):65-78.
The effect of biological response modifiers on macroscopic tumor growth and on tumor cell proliferation of a human renal cell carcinoma and a squamous cell carcinoma (hypopharynx) in nude mice has been studied. Tumor necrosis factor alpha (TNF-alpha) and interferon alpha (IFN-alpha) as well as granulocyte-macrophage colony-stimulating factor (GM-CSF) were applied either alone or in combination, and TNF-alpha was also combined with etoposide (ETP). TNF-alpha and IFN-alpha alone or in combination did not substantially affect the course of tumor growth, however, they did influence the pattern of tumor growth. There was also only a marginal effect on tumor cell proliferation. However, IFN-alpha protects the animals from tumor growth associated weight loss. ETP and ETP plus TNF-alpha leads to a deceleration of tumor growth, a decrease of the labeling index and to a significant decrease of the animal weight which indicates that the first two effects may be partly due to the toxicity of the treatment. GM-CSF modifies cell proliferation in a dose-dependent manner, i.e. stimulation at low doses and tendency to inhibition at higher doses. Although there is no substantial direct antineoplastic effect of the agents studied, the results make clear that indirect effects of therapeutic agents due to therapy induced cachexia should always be regarded. It is interesting that IFN-alpha has a protective effect against cachexia.
研究了生物反应调节剂对裸鼠体内人肾细胞癌和鳞状细胞癌（下咽）的宏观肿瘤生长及肿瘤细胞增殖的影响。单独或联合应用肿瘤坏死因子α（TNF-α）、干扰素α（IFN-α）以及粒细胞巨噬细胞集落刺激因子（GM-CSF），并且TNF-α还与依托泊苷（ETP）联合应用。单独或联合使用的TNF-α和IFN-α对肿瘤生长进程没有实质性影响，然而，它们确实影响了肿瘤生长模式。对肿瘤细胞增殖也仅有轻微影响。但是，IFN-α可保护动物免于因肿瘤生长而导致的体重减轻。ETP以及ETP加TNF-α导致肿瘤生长减速、标记指数降低以及动物体重显著下降，这表明前两种效应可能部分归因于治疗的毒性。GM-CSF以剂量依赖方式改变细胞增殖，即低剂量时刺激，高剂量时有抑制趋势。尽管所研究的药物没有实质性的直接抗肿瘤作用，但结果清楚表明，应始终考虑治疗药物因治疗引起的恶病质所产生的间接效应。有趣的是，IFN-α对恶病质具有保护作用。
