Hansen N J, Kristensen P, Lykke J, Mortensen K K, Clark B F
Department of Biostructural Chemistry, University of Aarhus, Denmark.
Biochem Mol Biol Int. 1995 Mar;35(3):461-5.
A fast, efficient and economical method for purification of DNA from various sources by the use of silica particles packed in homemade bead columns is described. The method is a further development of the method devised by Carter and Milton (1), improved by the introduction of a column operated with a vacuum system. The method is highly suitable for multiple sample handling in the preparation of DNA for sequencing and other purposes. Protocols are devised for plasmid minipreparation, gel elution and purification of DNA from solutions e.g. enzyme reactions.
本文描述了一种通过使用填充在自制磁珠柱中的硅胶颗粒从各种来源快速、高效且经济地纯化DNA的方法。该方法是卡特和米尔顿（1）设计的方法的进一步发展，并通过引入使用真空系统操作的柱子进行了改进。该方法非常适合在制备用于测序和其他目的的DNA时进行多样本处理。还设计了用于质粒小量制备、凝胶洗脱以及从溶液（如酶反应）中纯化DNA的方案。
