Manon S, Guérin M
Institut de Biochemie et de Génétique Cellulaires du Centre National de la Recherche Scientifique, Université de Bordeaux II, France.
Biochem Mol Biol Int. 1995 Mar;35(3):585-93.
Effect of Zn2+ and Cu2+ were studied on the spontaneous swelling of yeast mitochondria in potassium acetate. (i) Whatever the pH, Cu2+ stimulated the swelling. (ii) Zn2+ inhibited or stimulated the swelling depending both on pH of the medium and of its concentration: it induced an inhibition of the K+/H+ exchange at pH 6.8 and a stimulation at pH 7.7; at pH 7.4, Zn2+ inhibited the swelling at low concentration and stimulated the swelling at higher concentration. (iii) The thiol reagent mersalyl prevented the inhibition by Zn2+ whereas N-ethylmaleimide and 5,5'-dithiobis(2-nitrobenzoate) prevented the stimulation by Zn2+: this allowed to hypothesize that two distinct Zn(2+)-binding sites were involved in the inhibition and in the stimulation of the swelling of yeast mitochondria suspended in potassium acetate.
研究了Zn2+和Cu2+对酵母线粒体在醋酸钾溶液中自发肿胀的影响。(i) 无论pH值如何，Cu2+均会刺激肿胀。(ii) Zn2+对肿胀的抑制或刺激作用取决于培养基的pH值及其浓度：在pH 6.8时，它会抑制K+/H+交换，而在pH 7.7时则会刺激；在pH 7.4时，低浓度的Zn2+会抑制肿胀，而高浓度时则会刺激肿胀。(iii) 硫醇试剂汞撒利可防止Zn2+的抑制作用，但N-乙基马来酰亚胺和5,5'-二硫代双(2-硝基苯甲酸)可防止其刺激作用：由此推测，在醋酸钾中悬浮的酵母线粒体肿胀的抑制和刺激过程中涉及两个不同的Zn(2+)结合位点。
