Ariel M, Robinson E, McCarrey J R, Cedar H
Department of Cellular Biochemistry, Hebrew University Medical School, Jerusalem, Israel.
Nat Genet. 1995 Mar;9(3):312-5. doi: 10.1038/ng0395-312.
We have investigated the potential role of DNA methylation as a regulator of imprinted Xist expression in mouse preimplantation embryos. The active paternal allele was found to be unmodified in sperm at CpG loci near the 5' end of the gene transcription unit. In contrast, on the inactive maternal allele, these same sites are initially methylated in the oocyte and then remain modified in the early embryo. In the male germ line, these methyl moieties are removed during spermatogenesis, and this occurs before the programmed reactivation of Xist in the testis. This represents a clear-cut example of a potential methylation imprinting signal that is reprogrammable and gamete derived.
我们研究了DNA甲基化作为小鼠植入前胚胎中印迹Xist表达调节因子的潜在作用。发现活跃的父本等位基因在基因转录单元5'端附近的CpG位点上在精子中未被修饰。相比之下，在不活跃的母本等位基因上，这些相同的位点在卵母细胞中最初被甲基化，然后在早期胚胎中保持修饰状态。在雄性生殖系中，这些甲基部分在精子发生过程中被去除，并且这发生在睾丸中Xist程序性重新激活之前。这代表了一个可重新编程且源自配子的潜在甲基化印记信号的明确例子。
