Andersen O, Dehli A, Standal H, Giskegjerde T A, Karstensen R, Rørvik K A
Akvaforsk, Institute of Aquaculture Research, Aas, Norway.
Mol Mar Biol Biotechnol. 1995 Jun;4(2):164-70.
The ferritin heavy (H) and middle (M) subunit cDNAs were isolated from the Atlantic salmon (Salmo salar) liver. Full-length clones encoding the ferritin M subunit of 176 residues were obtained by screening of a liver cDNA library. The evolutionary conserved iron-responsive element (IRE) was identified in the upstream untranslated region. Ferritin H cDNA was cloned by running reverse transcription-polymerase chain reaction (RT-PCR) on salmon liver mRNA. The salmon ferritin H subunit of 177 residues showed 67% sequence identity with the M subunit. Northern blot analysis revealed ferritin H mRNA in the liver, gonads, head kidney, heart, and spleen, whereas M subunit mRNA was found almost exclusively in the gonads. Polyclonal antibodies against both salmon ferritin H and M were raised in rabbits.
从大西洋鲑鱼（Salmo salar）肝脏中分离出铁蛋白重链（H）和中链（M）亚基的cDNA。通过筛选肝脏cDNA文库获得了编码176个残基的铁蛋白M亚基的全长克隆。在5′非翻译区鉴定出进化保守的铁反应元件（IRE）。通过对鲑鱼肝脏mRNA进行逆转录-聚合酶链反应（RT-PCR）克隆了铁蛋白H cDNA。由177个残基组成的鲑鱼铁蛋白H亚基与M亚基的序列同一性为67%。Northern印迹分析显示肝脏、性腺、头肾、心脏和脾脏中有铁蛋白H mRNA，而M亚基mRNA几乎只在性腺中发现。用兔制备了抗鲑鱼铁蛋白H和M的多克隆抗体。
