Venance L, Cordier J, Monge M, Zalc B, Glowinski J, Giaume C
INSERM U114, Collège de France, Paris.
Eur J Neurosci. 1995 Mar 1;7(3):451-61. doi: 10.1111/j.1460-9568.1995.tb00341.x.
Intercellular communication mediated by gap junctions was investigated during oligodendrocyte differentiation in primary and secondary cell cultures from newborn and adult rats. Two types of communication were considered: ionic coupling and dye-coupling between similar oligodendrocytes selected at the same stage of differentiation (homotypic) and dye-coupling between oligodendrocytes and astrocytes (heterotypic). Intercellular diffusion of fluorescent probes and double whole-cell recordings were used to test the incidence of dye and ionic communication respectively. Progenitor cells, identified with A2B5 antibodies, were characterized by the absence of ionic and dye-coupling, whereas oligodendrocytes, identified with galactosylceramide antibodies, exhibited both types of communication. This homotypic coupling was inhibited by various uncoupling agents, but unaffected by treatments which increased the intracellular concentration of cAMP. In cocultures of astrocytes and oligodendrocytes, Lucifer yellow and sulphorhodamine B were exchanged in both directions. This heterotypic dye-coupling, which could be blocked by octanol, first appeared after 3 weeks in culture and increased to an incidence of 25% after 6 weeks, a developmental pattern comparable to homotypic dye-coupling between oligodendrocytes. In contrast, during the same period, progenitors and microglia were never observed to be dye-coupled with astrocytes.
在新生大鼠和成年大鼠原代及传代细胞培养的少突胶质细胞分化过程中，研究了由缝隙连接介导的细胞间通讯。考虑了两种通讯类型：处于相同分化阶段的相似少突胶质细胞之间的离子偶联和染料偶联（同型），以及少突胶质细胞与星形胶质细胞之间的染料偶联（异型）。分别使用荧光探针的细胞间扩散和双全细胞记录来测试染料通讯和离子通讯的发生率。用A2B5抗体鉴定的祖细胞的特征是不存在离子偶联和染料偶联，而用半乳糖基神经酰胺抗体鉴定的少突胶质细胞则表现出两种通讯类型。这种同型偶联受到各种解偶联剂的抑制，但不受增加细胞内cAMP浓度的处理的影响。在星形胶质细胞和少突胶质细胞的共培养中，荧光素黄和磺基罗丹明B双向交换。这种异型染料偶联可被辛醇阻断，在培养3周后首次出现，6周后发生率增加到25%，其发育模式与少突胶质细胞之间的同型染料偶联相当。相比之下，在同一时期，从未观察到祖细胞和小胶质细胞与星形胶质细胞有染料偶联。
