A rapid radioimmunoassay for human alpha-fetoprotein.

Our previous double antibody radioimmunoassay for human AFP was run at 4 degrees C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody complex. The modified radioimmunoassay was standardized against an international reference preparation of human AFP. Characteristics of this assay are discussed.