Kubushiro K, Tsukazaki K, Sakuma Y, Sakayori M, Yazawa S, Nozawa S
Department of Obstetrics and Gynecology, School of Medicine, Keio University.
Jpn J Cancer Res. 1995 Apr;86(4):361-7. doi: 10.1111/j.1349-7006.1995.tb03065.x.
In order to clarify the mechanism of the abnormal expression of Lewis(b) antigen, which was specific for uterine endometrial cancer tissue, the activities of alpha 1-->2fucosyltransferase, alpha 1-->3fucosyltransferase, and alpha 1-->4fucosyltransferase in normal endometrial tissues and uterine endometrial cancer tissues were determined. Further, an immunocytochemical study of the expression of blood group-related carbohydrate antigens in 6 cultured cell lines derived from various gynecologic malignant tumors was performed and the alpha 1-->2fucosyltransferase, alpha 1-->3fucosyltransferase, and alpha 1-->4fucosyltransferase activities of these cell lines were determined. Compared with normal endometrium, uterine endometrial cancer tissues showed significantly higher values of alpha 1-->2fucosyltransferase, alpha 1-->3fucosyltransferase, and alpha 1-->4fucosyltransferase activities. The specifically strong expression of type I carbohydrate chains, particularly the Lewis(b) antigen, was recognized in cultured cell lines derived from uterine endometrial cancer. Compared with those cell lines derived from uterine cervical cancer and ovarian cancer, the cultured cell lines derived from uterine endometrial cancer showed higher activities of alpha 1-->2fucosyltransferase and alpha 1-->4fucosyltransferase, which are enzymes related to the synthesis of Lewis(b) antigen. The cell lines derived from uterine endometrial cancer showed specifically high values of alpha 1-->4fucosyltransferase activity. These results suggest that the alpha 1-->2fucosyltransferase and alpha 1-->4fucosyltransferase activities, especially the alpha 1-->4fucosyltransferase activity, contribute to the abnormal expression of the Lewisb antigen in uterine endometrial cancer.
为阐明子宫子宫内膜癌组织特有的Lewis(b)抗原异常表达的机制，测定了正常子宫内膜组织和子宫子宫内膜癌组织中α1→2岩藻糖基转移酶、α1→3岩藻糖基转移酶和α1→4岩藻糖基转移酶的活性。此外，对6种源自各种妇科恶性肿瘤的培养细胞系中血型相关碳水化合物抗原的表达进行了免疫细胞化学研究，并测定了这些细胞系的α1→2岩藻糖基转移酶、α1→3岩藻糖基转移酶和α1→4岩藻糖基转移酶活性。与正常子宫内膜相比，子宫子宫内膜癌组织中α1→2岩藻糖基转移酶、α1→3岩藻糖基转移酶和α1→4岩藻糖基转移酶的活性值显著更高。在源自子宫子宫内膜癌的培养细胞系中，I型碳水化合物链，尤其是Lewis(b)抗原，呈现出特异性的强表达。与源自子宫颈癌和卵巢癌的细胞系相比，源自子宫子宫内膜癌的培养细胞系显示出更高的α1→2岩藻糖基转移酶和α1→4岩藻糖基转移酶活性，这两种酶与Lewis(b)抗原的合成有关。源自子宫子宫内膜癌的细胞系显示出特别高的α1→4岩藻糖基转移酶活性值。这些结果表明，α1→2岩藻糖基转移酶和α1→4岩藻糖基转移酶活性，尤其是α1→4岩藻糖基转移酶活性，促成了子宫子宫内膜癌中Lewis(b)抗原的异常表达。
