Sato A, Bo M, Otani T, Mochizuki M
Department of Obstetrics and Gynecology, Kobe University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1995 May;47(5):473-8.
To study the regulation of c-erbB, EFG and TGF-alpha in endometrium, we examined the expression of their mRNA in 5 normal endometrial and 5 endometrial carcinoma tissues by Northern blot and reverse transcription-polymerase chain reaction (RT-PCR). By Northern blot with 10 micrograms of total RNA, c-erbB mRNA was not detected in the normal endometrium, but it was detected in 3 samples of endometrial carcinoma tissues. On the other hand RT-PCR identified c-erbB mRNA in all the normal endometrium and endometrial carcinoma tissues by amplifying cDNA derived from c-erbB mRNA. EGF mRNA was detected by RT-PCR in normal endometrium except in the early follicular phase. It was detected in all cases of endometrial carcinoma tissues. TGF-alpha mRNA was also detected in all the normal and endometrial carcinoma tissues by RT-PCR. Our study suggests that an autocrine/paracrine mechanism of EGF may regulate the endometrial cycle. Because some endometrial carcinoma tissues express the c-erbB mRNA much more than normal endometrium, disruption of the autocrine/paracrine mechanism may trigger subsequent endometrial carcinogenesis.
为研究子宫内膜中c-erbB、表皮生长因子（EGF）和转化生长因子-α（TGF-α）的调控，我们采用Northern印迹法和逆转录-聚合酶链反应（RT-PCR）检测了5例正常子宫内膜组织和5例子宫内膜癌组织中它们的mRNA表达。用10微克总RNA进行Northern印迹分析时，在正常子宫内膜中未检测到c-erbB mRNA，但在3例子宫内膜癌组织样本中检测到了。另一方面，RT-PCR通过扩增源自c-erbB mRNA的cDNA，在所有正常子宫内膜和子宫内膜癌组织中均鉴定出了c-erbB mRNA。除卵泡早期外，RT-PCR在正常子宫内膜中检测到了EGF mRNA。在所有子宫内膜癌组织病例中均检测到了该mRNA。RT-PCR在所有正常和子宫内膜癌组织中也检测到了TGF-α mRNA。我们的研究表明，EGF的自分泌/旁分泌机制可能调节子宫内膜周期。由于一些子宫内膜癌组织中c-erbB mRNA的表达比正常子宫内膜高得多，自分泌/旁分泌机制的破坏可能引发随后的子宫内膜癌发生。
