Gough J M, Kemp D H
CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Queensland, Australia.
J Parasitol. 1995 Jun;81(3):341-9.
Enzyme cytochemistry was used to identify vesicles containing acid phosphatase in the midgut digestive cells of partially fed females of the cattle tick Boophilus microplus. The vesicles were elongated or tubular in shape and appeared to be involved with the digestion of host bloodmeal. In mature cells, they were sometimes in close contact with large endosomes, which contained host blood. The vesicles were identified as tubular lysosomes because their morphological and cytochemical characteristics were analogous to similar structures described in mammalian cells. This is the first report of such lysosomes in tick gut cells and suggests some parallels with the intracellular structures involved in the digestion process of mammalian cells. Acid phosphatase in tick gut was also assayed biochemically and was shown to be inhibited with 10 mM sodium fluoride. Cytochemistry showed that this inhibitor blocked activity within the cell and on the lumenal cell membrane.
利用酶细胞化学方法鉴定微小牛蜱部分取食雌蜱中肠消化细胞内含有酸性磷酸酶的囊泡。这些囊泡呈细长形或管状，似乎参与宿主血餐的消化。在成熟细胞中，它们有时与含有宿主血液的大型内体紧密接触。这些囊泡被鉴定为管状溶酶体，因为它们的形态和细胞化学特征与哺乳动物细胞中描述的类似结构相似。这是蜱肠道细胞中此类溶酶体的首次报道，表明与哺乳动物细胞消化过程中涉及的细胞内结构存在一些相似之处。还对蜱肠道中的酸性磷酸酶进行了生化分析，结果表明其被10 mM氟化钠抑制。细胞化学显示该抑制剂阻断了细胞内和腔面膜上的活性。
