Volpe B T, Wessel T C, Mukherjee B, Federoff H J
Department of Neurology and Neuroscience, Cornell University Medical School, White Plains, NY 10605, USA.
Neurosci Lett. 1995 Feb 17;186(2-3):157-60. doi: 10.1016/0304-3940(95)11310-s.
Transient forebrain ischemia in rodents caused internucleosomal DNA fragmentation that appeared in the striatum 24 h after reperfusion, and in the hippocampus 72 h after reperfusion. Gel electrophoresis and an in situ technique to label 3' termini of endonuclease generated DNA fragments demonstrated similar temporal patterns. These data show that endonuclease activation accompanies the demise of selectively vulnerable neurons following transient forebrain ischemia.
啮齿动物短暂性前脑缺血会导致核小体间DNA片段化，其在再灌注后24小时出现在纹状体中，在再灌注后72小时出现在海马体中。凝胶电泳和一种标记核酸内切酶产生的DNA片段3'末端的原位技术显示出相似的时间模式。这些数据表明，核酸内切酶激活伴随着短暂性前脑缺血后选择性易损神经元的死亡。
