[涡虫再生过程中表达的同源异型框基因片段的克隆]
[The cloning of fragments of homeobox genes expressed during planarian regeneration].
作者信息
Luk'ianov K A, Tarabykin V S, Potapov V K, Bekman E P, Luk'ianov S A
出版信息
Ontogenez. 1994 Nov-Dec;25(6):28-32.
The polymerase chain reaction with degenerate primers corresponding to the most conservative amino acids 16-21 (ELEKEF) and 49-54 (WFQNRR) of the Antennapedia class homeodomain was used for the amplification of cDNA from regenerating planarians (asexual race of Dugesia tigrina). A total of six new Antennapedia-like homeobox sequences, designated Dutarh-1-Dutarh-6 (Dugesia tigrina asexual race homeobox gene), were obtained. Their comparison with other homeobox genes using a "Genebee" software (the EMBL Data Library) showed that all sequences except Dutarh-6 belong to the Antennapedia class. Dutarh-6 is closely related to a recently described novel homeobox gene subfamily that includes mouse mesodermal homeobox genes Mox-1 and Mox-2 and rat homeobox gene Gax.
利用与触角足类同源异型结构域最保守的氨基酸16 - 21（ELEKEF）和49 - 54（WFQNRR）对应的简并引物进行聚合酶链反应，以扩增来自再生涡虫（三角真涡虫无性系）的cDNA。总共获得了六个新的类触角足类同源异型框序列，命名为Dutarh - 1 - Dutarh - 6（三角真涡虫无性系同源异型框基因）。使用“Genebee”软件（EMBL数据库）将它们与其他同源异型框基因进行比较，结果表明除Dutarh - 6外的所有序列都属于触角足类。Dutarh - 6与最近描述的一个新的同源异型框基因亚家族密切相关，该亚家族包括小鼠中胚层同源异型框基因Mox - 1和Mox - 2以及大鼠同源异型框基因Gax。
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