Montan P G, Biberfeld P J, Scheynius A
Department of Ophthalmology, St. Eriks Hospital, Stockholm, Sweden.
Ophthalmology. 1995 May;102(5):725-32. doi: 10.1016/s0161-6420(95)30962-1.
Vernal keratoconjunctivitis (VKC) often is associated with atopy but a considerable part of the patients lack evidence of specific hypersensitivity. An immunocytochemical study was undertaken with the aim of detecting possible differences in the tissue inflammatory response between atopic and nonatopic subjects with VKC.
Conjunctival biopsy specimens from six atopic and three nonatopic subjects with VKC (age range, 7-17 years) and eight healthy control subjects (age range, 3-15 years) were analyzed with a panel of monoclonal and polyclonal antibodies.
No distinct differences in cell counts between atopic and nonatopic subjects with VKC were observed. Vernal keratoconjunctivitis specimens as a whole showed higher counts than control specimens for CD3+, CD4+, HLA-DR+, CD38+, EG2+, CD68+, IgE+, FC epsilon RI+, IgA+, IgG+, and IgM+ cells in the substantia propria; and for CD1a+, IgE+, and EG2+ cells in the epithelium.
Because the number of cells expressing CD4, EG2, IgE, and the high-affinity receptor for IgE, all of which are considered relevant in infiltrates of allergic conditions, were similar in atopic and nonatopic subjects, the authors conclude that the role of allergen-specific IgE sensitization in the pathogenesis of VKC remains unclear.
春季角结膜炎（VKC）常与特应性相关，但相当一部分患者缺乏特异性超敏反应的证据。进行了一项免疫细胞化学研究，旨在检测患有VKC的特应性和非特应性受试者之间组织炎症反应的可能差异。
用一组单克隆和多克隆抗体分析了6名患有VKC的特应性受试者、3名患有VKC的非特应性受试者（年龄范围7 - 17岁）以及8名健康对照受试者（年龄范围3 - 15岁）的结膜活检标本。
患有VKC的特应性和非特应性受试者之间的细胞计数未观察到明显差异。总体而言，春季角结膜炎标本在固有层中CD3 +、CD4 +、HLA - DR +、CD38 +、EG2 +、CD68 +、IgE +、FcεRI +、IgA +、IgG +和IgM +细胞的计数高于对照标本；在上皮中CD1a +、IgE +和EG2 +细胞的计数高于对照标本。
由于表达CD4、EG2、IgE以及IgE高亲和力受体的细胞数量在特应性和非特应性受试者中相似，而这些细胞在过敏性疾病浸润中均被认为具有相关性，因此作者得出结论，变应原特异性IgE致敏在VKC发病机制中的作用仍不清楚。
