α1/α2/DNA三元复合物的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction studies of an a1/alpha 2/DNA ternary complex.

作者信息

Li T, Stark M, Johnson A D, Wolberger C

机构信息

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

出版信息

Proteins. 1995 Feb;21(2):161-4. doi: 10.1002/prot.340210210.

DOI:10.1002/prot.340210210

PMID:7777491

Abstract

Crystals have been obtained of a ternary complex containing the yeast a1/alpha 2 homeodomain heterodimer bound to a 21-base pair DNA site containing two 5' overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P6(1) or P6(5) with a = b = 133 A, c = 45.4 A. Crystals that are flash-frozen at -179 degrees C diffract to 2.7 A along the c-axis and to 2.4 A in perpendicular directions. The crystals contain one protein-DNA complex in the crystallographic asymmetric unit.

摘要

已获得一种三元复合物的晶体，该复合物包含与一个21碱基对的DNA位点结合的酵母a1/α2同源域异二聚体，该DNA位点两端各有两个5'突出碱基。这些晶体由六胺钴生长而成，形成于空间群P6(1)或P6(5)中，a = b = 133 Å，c = 45.4 Å。在-179℃下快速冷冻的晶体沿c轴方向的衍射分辨率为2.7 Å，垂直方向为2.4 Å。晶体在晶体学不对称单元中包含一个蛋白质-DNA复合物。