Peng X X, Currin R T, Thurman R G, Lemasters J J
Department of Cell Biology, University of North Carolina at Chapel Hill 27599-7090, USA.
Transplantation. 1995 Jun 15;59(11):1537-41.
Previously, pentoxifylline treatment of graft recipients was shown to protect against liver graft failure from storage/reperfusion injury after orthotopic rat liver transplantation. To determine whether pentoxifylline also protects against normothermic ischemia/reperfusion injury to liver, we induced lobar ischemia in rats followed by reflow and partial hepatectomy of the noninvolved liver. In rats receiving pentoxifylline 2 hr before surgery and then twice daily for 5 days, the 1-week survival rate more than doubled from 25% to 67% (P < 0.05). Liver enzymes (alanine transaminase, aspartate transaminase, and lactate dehydrogenase) in the serum and liver necrosis evaluated histologically were also significantly reduced in the pentoxifylline-treated rats (P < 0.01). Hepatic ischemia/reperfusion increased leukocyte infiltration into the lungs, and pentoxifylline tended to reduce this lung injury (P = 0.06). These results show that pentoxifylline treatment reduces hepatic injury and improves survival after normothermic ischemia and reperfusion.
先前的研究表明，在大鼠原位肝移植后，己酮可可碱治疗可保护移植受体免受肝脏移植因保存/再灌注损伤而导致的肝移植失败。为了确定己酮可可碱是否也能保护肝脏免受常温缺血/再灌注损伤，我们对大鼠进行叶状肝缺血，随后进行再灌注，并对未受累肝脏进行部分肝切除术。在手术前2小时接受己酮可可碱治疗且随后连续5天每天接受两次治疗的大鼠中，1周生存率从25%增加了一倍多，达到67%（P<0.05）。在接受己酮可可碱治疗的大鼠中，血清中的肝酶（丙氨酸转氨酶、天冬氨酸转氨酶和乳酸脱氢酶）以及组织学评估的肝坏死也显著降低（P<0.01）。肝脏缺血/再灌注增加了白细胞向肺部的浸润，己酮可可碱倾向于减轻这种肺部损伤（P = 0.06）。这些结果表明，己酮可可碱治疗可减轻常温缺血和再灌注后的肝脏损伤并提高生存率。
