Zakirov M M, Petrov A B, Burkhanov S A, Vartanian Iu P, Torchilin V P, Trubetskoĭ V S, Koshkina N V, Dmitriev B A, L'vov V L
Zh Mikrobiol Epidemiol Immunobiol. 1995 Jan-Feb(1):49-53.
Immune response to lipooligosaccharide (LOS), isolated from group B N. meningitidis cell wall, was studied after its incorporation into liposomes. The adjuvant effect produced by liposomal LOS, more pronounced in comparison with that produced by native LOS, was observed after the injection of the preparation by different routes (intravenous, intraperitoneal and subcutaneous injections), but the highest level of response was achieved after the intravenous and intraperitoneal injections of the preparation. Experiments aimed at the study of the dynamics of plaque-forming cells and the level of antibodies after intraperitoneal immunization of CBA mice with LOS revealed that immune response to liposomal LOS was more intense and prolonged than that to the free antigen. Liposomal LOS induced genetically nonrestricted and T-independent immune response. This fact was confirmed in experiments on mice having different haplotypes, such as CBA (k), C57BL/6 (b), BALB/c (d) and in nude mice with congenital thymic aplasia. In addition, liposomal LOS induced mainly the accumulation of IgM and IgG and did not produce the effect of immunological memory.
研究了从B群脑膜炎奈瑟氏球菌细胞壁分离的脂寡糖（LOS）掺入脂质体后的免疫反应。脂质体LOS产生的佐剂效应比天然LOS产生的佐剂效应更明显，通过不同途径（静脉内、腹膜内和皮下注射）注射制剂后均可观察到，但在静脉内和腹膜内注射制剂后反应水平最高。旨在研究CBA小鼠腹膜内用LOS免疫后噬斑形成细胞的动力学和抗体水平的实验表明，对脂质体LOS的免疫反应比对游离抗原的免疫反应更强烈且持续时间更长。脂质体LOS诱导基因非限制性和T细胞非依赖性免疫反应。这一事实在具有不同单倍型的小鼠实验中得到证实，如CBA（k）、C57BL/6（b）、BALB/c（d）以及先天性胸腺发育不全的裸鼠。此外，脂质体LOS主要诱导IgM和IgG的积累，并未产生免疫记忆效应。
