细胞外钙对慢性肾衰竭继发性甲状旁腺增生和腺瘤患者甲状旁腺分散细胞中甲状旁腺激素相关蛋白释放的抑制作用。

Inhibition of parathyroid hormone-related protein release by extracellular calcium in dispersed cells from human parathyroid hyperplasia secondary to chronic renal failure and adenoma.

作者信息

Matsushita H, Hara M, Honda K, Kuroda M, Usui M, Nakazawa H, Hara S, Shishiba Y

机构信息

Department of Pathology, Toranomon Hospital, Tokyo, Japan.

出版信息

Am J Pathol. 1995 Jun;146(6):1521-8.

PMID:7778690

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1870896/

Abstract

The relationship between parathyroid hormone-related protein (PTHrP) release from parathyroid cells and extracellular calcium ion concentration was investigated in three cases of parathyroid hyperplasia secondary to chronic renal failure and in four cases of parathyroid adenoma. Amounts of PTHrP released from individual parathyroid cells dispersed from surgical specimens were estimated by cell immunoblot assay. Parathyroid cells from both hyperplasias and adenomas showed significant suppression in the release of PTHrP with increase in extracellular calcium ions, but the amounts of PTHrP released from adenoma cells were significantly larger than from hyperplasia cells. The maximal value for PTHrP released within 120 minutes from adenoma cells was 2.91 +/- 2.11 x 10(-2) fmol/cell ([Ca2+], 0.4 mmol/L), and the minimal value was 1.32 +/- 0.35 x 10(-2) fmol/cell ([Ca2+], 2.0 mmol/L). On the other hand, the maximal value for PTHrP released from hyperplasia cells was 1.79 +/- 1.56 x 10(-2) fmol/cell ([Ca2+], 0.4 mmol/L), and the minimal value was 0.32 +/- 0.19 x 10(-2) fmol/cell ([Ca2+], 2.0 mmol/L). These results demonstrate actual release of PTHrP from abnormal parathyroid tissues into the extracellular space with the response to extracellular calcium ions depending on the cell status. Given the lack of definite histological criteria to differentiate between hyperplasias and adenomas in the parathyroid gland, the presently demonstrated significant difference in the ability to release PTHrP is important in pointing to parathyroid hyperplasia secondary to chronic renal failure as a distinct pathological entity separate from parathyroid adenoma.

摘要

在3例慢性肾衰竭继发甲状旁腺增生及4例甲状旁腺腺瘤患者中，研究了甲状旁腺细胞释放甲状旁腺激素相关蛋白（PTHrP）与细胞外钙离子浓度之间的关系。通过细胞免疫印迹分析估算手术标本中单个分散甲状旁腺细胞释放的PTHrP量。增生和腺瘤的甲状旁腺细胞均显示，随着细胞外钙离子浓度增加，PTHrP释放受到显著抑制，但腺瘤细胞释放的PTHrP量显著高于增生细胞。腺瘤细胞在120分钟内释放PTHrP的最大值为2.91±2.11×10⁻² fmol/细胞（[Ca²⁺]，0.4 mmol/L），最小值为1.32±0.35×10⁻² fmol/细胞（[Ca²⁺]，2.0 mmol/L）。另一方面，增生细胞释放PTHrP的最大值为1.79±1.56×10⁻² fmol/细胞（[Ca²⁺]，0.4 mmol/L），最小值为0.32±0.19×10⁻² fmol/细胞（[Ca²⁺]，2.0 mmol/L）。这些结果表明，异常甲状旁腺组织实际向细胞外空间释放PTHrP，且对细胞外钙离子的反应取决于细胞状态。鉴于缺乏明确的组织学标准来区分甲状旁腺的增生和腺瘤，目前所证明的PTHrP释放能力的显著差异对于指出慢性肾衰竭继发甲状旁腺增生是与甲状旁腺腺瘤不同的独特病理实体具有重要意义。