白血病抑制因子（LIF）增强了由人绒毛膜促性腺激素（hCG）介导的滋养层细胞分化。

Leukemia inhibitory factor (LIF) enhances trophoblast differentiation mediated by human chorionic gonadotropin (hCG).

作者信息

Sawai K, Azuma C, Koyama M, Ito S, Hashimoto K, Kimura T, Samejima Y, Nobunaga T, Saji F

机构信息

Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

出版信息

Biochem Biophys Res Commun. 1995 Jun 6;211(1):137-43. doi: 10.1006/bbrc.1995.1788.

DOI:10.1006/bbrc.1995.1788

PMID:7779078

Abstract

We investigated the effect of LIF on the differentiation of trophoblasts. Isolated cytotrophoblasts were cultured with and without LIF and cell smears were immunocytochemically analyzed, using anti-hCG antibody. The percentage of differentiated trophoblasts stimulated by 10ng/ml of LIF was about 2.5-fold that in the control culture. The effect of LIF in inducing the differentiation of cytotrophoblasts to syncytiotrophoblasts was dose-dependent. The same effect was shown when hCG was added to the medium. This enhancing effect of LIF on trophoblast differentiation was blocked by adding anti-hCG antibody to the culture system. These results indicate that LIF enhanced trophoblast differentiation by stimulating hCG production in trophoblasts, and not by exerting a direct effect on the trophoblasts.

摘要

我们研究了白血病抑制因子（LIF）对滋养层细胞分化的影响。将分离出的细胞滋养层细胞分别在添加和不添加LIF的条件下进行培养，并使用抗人绒毛膜促性腺激素（hCG）抗体对细胞涂片进行免疫细胞化学分析。10纳克/毫升的LIF刺激下分化的滋养层细胞百分比约为对照培养中的2.5倍。LIF诱导细胞滋养层细胞分化为合体滋养层细胞的作用呈剂量依赖性。向培养基中添加hCG时也显示出相同的效果。通过向培养系统中添加抗hCG抗体，可阻断LIF对滋养层细胞分化的这种增强作用。这些结果表明，LIF通过刺激滋养层细胞中hCG的产生来增强滋养层细胞分化，而不是对滋养层细胞产生直接作用。