Saint-Jeannet J P, Karavanov A A, Dawid I B
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Int J Dev Biol. 1994 Dec;38(4):605-11.
In an attempt to document at the molecular level the behavior of mesodermal cells in Keller explant preparation, we have analyzed the time course of expression of four molecular markers of mesoderm gsc, Xbra, Xnot and XLIM-1. Our findings demonstrate that, (i) all mesodermal markers tested were expressed in the explants, but patterning of the mesoderm appeared incomplete; (ii) during convergence and extension of the explants, mesodermal cells did not invade the ectodermal tissue at any time tested, supporting the view that mesoderm establishes exclusively planar contacts with the ectoderm in this preparation; (iii) planar contacts were not sufficient to promote the neural expression of XLIM-1 protein in these explants.
为了在分子水平上记录中胚层细胞在凯勒外植体制备中的行为，我们分析了中胚层的四个分子标记物gsc、Xbra、Xnot和XLIM-1的表达时间进程。我们的研究结果表明：(i) 所有测试的中胚层标记物都在外植体中表达，但中胚层的模式似乎不完整；(ii) 在组织块的汇聚和延伸过程中，在所测试的任何时间，中胚层细胞都没有侵入外胚层组织，这支持了在这种制备方法中中胚层仅与外胚层建立平面接触的观点；(iii) 平面接触不足以促进这些外植体中XLIM-1蛋白的神经表达。
