Patek C, Head M, Clayton R
Division of Biological Sciences, University of Edinburgh, Scotland, United Kingdom.
Int J Dev Biol. 1994 Dec;38(4):717-24.
This two part study continues and extends our examination of the effects of age and of genetically determined intrinsic growth rate on the overall protein composition of chick lens epithelia, lens fiber masses and whole lenses. Water-soluble proteins were analyzed by SDS-polyacrylamide and 2-dimensional gel electrophoresis. First, detailed age-related changes in protein expression between day 4 of embryonic development and the 8-week adult stage are described for one normal chick strain. Secondly, comparisons are made between day-old post-hatch chicks of four different genotypes: two genetically unrelated chick strains with a high growth rate and propensity for hyperplasia of the lens epithelium and two unrelated slow-growing strains, both with normal lens morphology. We find that the beta/delta-crystallin ratio in lens epithelia and fiber masses is higher in both the slow- than in both the fast-growing strains. The data emphasizes the importance of quantitative and non-coordinate changes in crystallin polypeptide expression during lens growth and development, and implicates growth rate as a modifier of the pattern of crystallin expression.
这项分为两部分的研究继续并扩展了我们对年龄以及基因决定的内在生长速率对鸡晶状体上皮、晶状体纤维团和整个晶状体的整体蛋白质组成的影响的研究。通过SDS-聚丙烯酰胺和二维凝胶电泳分析水溶性蛋白质。首先，描述了一个正常鸡品系在胚胎发育第4天到8周龄成年阶段之间蛋白质表达的详细年龄相关变化。其次，对四种不同基因型的刚孵出一天的雏鸡进行了比较：两种生长速率高且晶状体上皮有增生倾向的遗传无关鸡品系，以及两种生长缓慢且晶状体形态正常的无关品系。我们发现，在晶状体上皮和纤维团中，慢生长品系的β/δ-晶状体蛋白比率高于快生长品系。这些数据强调了晶状体生长和发育过程中晶状体蛋白多肽表达的定量和非协同变化的重要性，并表明生长速率是晶状体蛋白表达模式的一个调节因素。
