核质蛋白介导的加州贻贝精子染色质解聚。加州贻贝中具有精子核解聚活性的类核质蛋白的鉴定及部分特性分析。

Nucleoplasmin-mediated decondensation of Mytilus sperm chromatin. Identification and partial characterization of a nucleoplasmin-like protein with sperm-nuclei decondensing activity in Mytilus californianus.

作者信息

Rice P, Garduño R, Itoh T, Katagiri C, Ausio J

机构信息

Department of Biochemistry and Microbiology, University of Victoria, BC, Canada.

出版信息

Biochemistry. 1995 Jun 13;34(23):7563-8. doi: 10.1021/bi00023a001.

DOI:10.1021/bi00023a001

PMID:7779801

Abstract

We have been able to induce sperm nuclear decondensation in the mussel Mytilus californianus (mollusc) using either egg extracts or pure nucleoplasmin from Xenopus (amphibian). The nuclear decondensation involves removal of the sperm nuclear basic proteins (SNBPs) which bind to nucleoplasmin. An attempt has been made to isolate an ooplasmic factor from Mytilus with a similar sperm-chromatin decondensing activity. An acidic, thermostable protein with a molecular mass of 58,000 has been purified and partially characterized.

摘要

我们已经能够使用卵提取物或非洲爪蟾（两栖动物）的纯核质蛋白，诱导加州贻贝（软体动物）的精子核去浓缩。核去浓缩涉及去除与核质蛋白结合的精子核碱性蛋白（SNBPs）。人们尝试从贻贝中分离出一种具有类似精子染色质去浓缩活性的卵质因子。一种分子量为58,000的酸性、热稳定蛋白已被纯化并进行了部分表征。