Nogués A, García M, Rivas C, Ruiz A, Falguera M, Puig T
Servicio de Análisis Clínicos-Microbiología, Hospital Arnau de Vilanova, Universidad de Lleida.
Enferm Infecc Microbiol Clin. 1995 May;13(5):288-91.
Sensitivity and specificity of latex agglutination test on samples obtained by transthoracic needle aspiration was evaluated for diagnosis of pneumococcal pneumonia by using the polymerase chain reaction as reference method.
Samples from 29 patients with community acquired pneumonia were processed for culture, antigen detection and polymerase chain reaction. The latex agglutination test was performed with a reagents kit (Slidex méningite kit, BioMérieux, France) using the procedure recommended by the manufacturer. The polymerase chain reaction was carried out by using two primers: Pn2x up and Pn2x down that amplify the PBP2x gene of Streptococcus pneumoniae.
Culture was positive in 5 patients, antigen detection in 15 and polymerase chain reaction in 14 patients. The specificity of latex agglutination test was 93% compared with polymerase chain reaction as reference method.
The pneumococcal antigen detection by latex agglutination test is as sensitive as the polymerase chain reaction, it seems to be highly specific, and it is rapid and easy to perform.
以聚合酶链反应作为参考方法，评估经胸针吸获取的样本进行乳胶凝集试验诊断肺炎球菌肺炎的敏感性和特异性。
对29例社区获得性肺炎患者的样本进行培养、抗原检测和聚合酶链反应。使用试剂试剂盒（法国生物梅里埃公司的Slidex脑膜炎试剂盒）按照制造商推荐的程序进行乳胶凝集试验。聚合酶链反应采用两种引物：Pn2x上游引物和Pn2x下游引物，扩增肺炎链球菌的PBP2x基因。
5例患者培养阳性，15例抗原检测阳性，14例聚合酶链反应阳性。以聚合酶链反应作为参考方法，乳胶凝集试验的特异性为93%。
乳胶凝集试验检测肺炎球菌抗原与聚合酶链反应一样敏感，似乎具有高度特异性，且快速、易于操作。
