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The in vitro culture of Onchocerca spp.: I. Preliminary observations on the development of the second to the third stage larvae.

作者信息

Hagen H E, Grunewald J, Ham P J

机构信息

Department of Biological Sciences, University of Keele, Staffordshire, UK.

出版信息

Appl Parasitol. 1995 Feb;36(1):47-52.

PMID:7780449
Abstract

Second stage larvae of Onchocerca lienalis and O. gutturosa were induced to moult successfully to the third stage in vitro. Following development in their vectors Simulium ornatum s.l. and Culicoides nubeculosus the second stage larvae were kept in a cell free culture system. At least 25% of the second stage larvae of either Onchocerca species completed the moult successfully although no feeder layers had been used. In the absence of CO2 (5% in air) the number of the second stage larvae moulting successfully to the infective stage dropped to 6 and 9% respectively.

摘要

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